Fusion expression and anti-Aspergillus flavus activity of a novel inhibitory protein DN-AflR

Expression of aflR, veA and laeA as regulators of aflatoxins and cyclopiazonic acid biosynthesis pathway in Aspergillus flavus

In this study, the production of aflatoxin B1 (AFB1) and cyclopiazonic acid (CPA) was investigated in toxigenic and non-toxigenic Aspergillus flavus with respect to expression of aflR, veA and laeA genes that are involved to toxins production. A. flavus strains were cultured in YES broth at 28 °C for 4 days and the presence of (AFB1) and (CPA) was confirmed and measured by TLC and HPLC. The exp...

Detection of Aflr Gene and Toxigenicity of Aspergillus flavus Group Isolated from Patients with Fungal Sinusitis

Background: Aspergillus flavus is the second most important Aspergillus species causing human infections particularly fungal sinusitis. Since little is known about aflatoxin producing ability of clinical isolates, this study was undertaken to detect the aflatoxigenic isolates amongst these isolates. Methods: A total of 23 isolates of A. spp. which were recovered from patients proved to have fun...

Silencing of the aflatoxin gene cluster in a diploid strain of Aspergillus flavus is suppressed by ectopic aflR expression.

Aflatoxins are toxic secondary metabolites produced by a 70-kb cluster of genes in Aspergillus flavus. The cluster genes are coordinately regulated and reside as a single copy within the genome. Diploids between a wild-type strain and a mutant (649) lacking the aflatoxin gene cluster fail to produce aflatoxin or transcripts of the aflatoxin pathway genes. This dominant phenotype is rescued in d...

Nonfunctionality of Aspergillus sojae aflR in a strain of Aspergillus parasiticus with a disrupted aflR gene.

Aspergillus sojae belongs to the Aspergillus section Flavi but does not produce aflatoxins. The functionality of the A. sojae aflR gene (aflRs) was examined by transforming it into an DeltaaflR strain of A. parasiticus, derived from a nitrate-nonutilizing, versicolorin A (VERA)-accumulating strain. The A. parasiticus aflR gene (aflRp) transformants produced VERA, but the aflRs transformants did...

Development of a Novel Inhibitory Chimeric Anti-HER2 Monoclonal Antibody